In-Fusion PCR Cloning System
I received a flyer for Clontech’s In-Fusion PCR Cloning System in the mail. I do try and scan the junk mail I receive, looking for the jewel in the junk. In-Fusion is a method of cloning that uses regions of engineered homology on your PCR generated insert and vector of choice to ligate without cutting your PCR fragment. Interesting. Maybe. Looking at the “time saved” flow chart. 30 minute ligation insead of 16 hours! That saves an entire day….if you still ligate overnight. In fairness, a footnote (in small print) reads “newer 5 min T4 DNA ligase reactions could be used”. The real laugher is provided by the jokers in advertising when they comapare In-Fusion’s cloning efficiency with T4 DNA ligase’s. In-Fusion, obviously, is rated “High” (surprise) for both .1 to 4 kb and 4 kb to 10 kb inserts. T4 DNA ligase PCR Cloning? No data available! Really? No data available? In-Fusion is better, much better when we compare it to “No data available”.
Clontech looks like a contender for the BioTech Bullshitter award for 2008!
Posted on Friday, February 8th, 2008 at 12:29 am Categorized as:General You can leave a response, or trackback from your own site.
