What’s In A Kit?
Wednesday, August 30th, 2006Occasionally I’ll buy a kit and there isn’t much information available about the included reagents. However in a recent email from Stratagene I noticed a link which led me to the following information about one of their most popular kits.
Stratagene’s Quickchange Mutagenesis Kit:
Contains: PfuUltra™ High Fidelity DNA polymerase, 10X reaction buffer, dNTP mix, Dpn I restriction enzyme, QuikChange control plasmid and control primers, XL-1 supercompetent cells, pUC18 control plasmid.
Save yourself some money and buy the reagents individually. There really isn’t any reason to pay an inflated price for some pUC18 and competent cells.
PS:I have never bought competent cells that transformed with a higher efficiency than those I made in the lab. On the rare occasion I’ve needed supercompetent cells I made electrocompetent cells (wash bacteria in water-how hard is that?).
Good luck and happy mutating!
