TrypLE
Invitrogen has a product called TrypLE, a recombinant enzyme obtained from microbial fermentation. This is a cell dissociation enzyme that is stable at room temperature for four months. The idea is that it works just like trypsin only it’s slightly more expensive. I’m not sure really what the advantage is of this product but if someone has tried it out and has an opinion, let me know!
Posted on Tuesday, November 29th, 2005 at 5:22 pm Categorized as:General You can leave a response, or trackback from your own site.
December 19th, 2005 at 12:40 am
Just came across this post: earlier this year, I was trying TrypLE as a replacement for 0.05% trypsin for human marrow-derived stromal cells (look/grow like fibroblasts). It worked well in small and big flasks. More expensive though (worth it for us, thinking about clinical products).