Novagen and Lab Rats
Wednesday, May 11th, 2005After waxing lyrical about the NEB Catalogue I’ve found that Novagen have some really useful resources too. In my line of work we try to express proteins that have as little vector sequence as possible, so use an in-house vector that is essentially pBR322 with a T7 promoter, Shine-Dalgarno and TATA boxes and a useful multiple cloning site. NdeI and NcoI are there (CATATG, CCATGG, remember) as well, meaning we can start translating at the first methionine codon without all that tedious mucking about in hyperspacegetting the frame right, minimizing extraneous linker residues, etc., etc.
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